Simultaneous Determination of Acetaminophen (Paracetamol) and Ascorbic Acid in Pharmaceutical Formulations by LC Coupled to a Screen Printed Carbon Based Amperometric Detector


Sarakbi A., Aydogmus Z. , Sidali T., Gokce G., Kauffmann J.

ELECTROANALYSIS, vol.23, no.1, pp.29-36, 2011 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 1
  • Publication Date: 2011
  • Doi Number: 10.1002/elan.201000494
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.29-36
  • Keywords: Acetaminophen, Screen printed electrodes, Monolithic column, Nafion modified electrodes, PERFORMANCE LIQUID-CHROMATOGRAPHY, VOLTAMMETRIC DETERMINATION, ELECTROCHEMICAL DETERMINATION, SENSITIVE DETECTION, MONOLITHIC COLUMNS, ELECTRODE, SENSOR, FILM, OXIDATION, CAFFEINE

Abstract

The assay of acetaminophen (APAP) and ascorbic acid (AA) was performed by liquid chromatography (LC) with amperometric detection at a screen printed carbon based electrode poised at +0.6 V versus Ag pseudoreference electrode. A microparticle based conventional RP-C18 and a monolithic RP-C18 column were studied comparatively for the analytes separation using a mobile phase based on 0. 1 M phosphate buffer-methanol 91: 9 (v/v) at pH 6.5. The linear calibration range for both compounds was comprised within 2 x 10(-7)-1 x 10(-4) M. Limit of detection (LOD) for APAP and AA was 2 x 10(-7) and 5 x 10(-7) M, respectively and the limit of quantification (LOQ) was 5. 10(-7)and 1 x 10(-6) M, respectively. Square wave voltammetry at a Nafion modified glassy carbon electrode was developed and applied to the determination of APAP in order to validate the results obtained by the monolithic LC method for drug formulations.