Simultaneous Determination of Acetaminophen (Paracetamol) and Ascorbic Acid in Pharmaceutical Formulations by LC Coupled to a Screen Printed Carbon Based Amperometric Detector


Sarakbi A., Aydogmus Z., Sidali T., Gokce G., Kauffmann J.

ELECTROANALYSIS, vol.23, no.1, pp.29-36, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 1
  • Publication Date: 2011
  • Doi Number: 10.1002/elan.201000494
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.29-36
  • Keywords: Acetaminophen, Screen printed electrodes, Monolithic column, Nafion modified electrodes, PERFORMANCE LIQUID-CHROMATOGRAPHY, VOLTAMMETRIC DETERMINATION, ELECTROCHEMICAL DETERMINATION, SENSITIVE DETECTION, MONOLITHIC COLUMNS, ELECTRODE, SENSOR, FILM, OXIDATION, CAFFEINE
  • Istanbul University Affiliated: No

Abstract

The assay of acetaminophen (APAP) and ascorbic acid (AA) was performed by liquid chromatography (LC) with amperometric detection at a screen printed carbon based electrode poised at +0.6 V versus Ag pseudoreference electrode. A microparticle based conventional RP-C18 and a monolithic RP-C18 column were studied comparatively for the analytes separation using a mobile phase based on 0. 1 M phosphate buffer-methanol 91: 9 (v/v) at pH 6.5. The linear calibration range for both compounds was comprised within 2 x 10(-7)-1 x 10(-4) M. Limit of detection (LOD) for APAP and AA was 2 x 10(-7) and 5 x 10(-7) M, respectively and the limit of quantification (LOQ) was 5. 10(-7)and 1 x 10(-6) M, respectively. Square wave voltammetry at a Nafion modified glassy carbon electrode was developed and applied to the determination of APAP in order to validate the results obtained by the monolithic LC method for drug formulations.