Metagenomic data analysis and characterization for the inhibitory effect of a novel Serratia fonticola strain against fungicide-resistant Botrytis cinerea
9. Uluslararası Moleküler Biyoloji ve Bitoteknoloji Kongresi, Kars, Turkey, 6 - 10 December 2020, vol.1, no.1, pp.107, (Summary Text)
- Publication Type: Conference Paper / Summary Text
- Volume: 1
- City: Kars
- Country: Turkey
- Page Numbers: pp.107
- Open Archive Collection: AVESIS Open Access Collection
- Istanbul University Affiliated: Yes
Abstract
Botrytis cinerea is a plant pathogenic fungi showing rapidly development of fungicide resistance in cultivation
fields and, changes its own genetic structure. Biocontrol may also reduce negative effects of toxic chemicals,
which is harmfull to environment and human healthy that is public concern besides its inhibitory effect on the
pathogen growth. A novel Gram (-) bacterial strain isolated from soil dispersed on agar plates treated with
phenol on 2 days old colonies was used to determine its antibiosis effect on spore suspension of Botrytis cinerea.
The studies showed restricted growth of the fungi spore around the phenol treated colonies. Afterwards, the
selected colonies showing the highest antibiosis effect tested using double-sheet agar bio-assay with the highest
and stable suppression on pathogen growth were used. Optimal concentration (CFU/ ml) was determined
according to spectrophotometric analysis. MIC concentration and inhibition percentage (%) indicated by 85%
inhibition ratio after 3 dpi and 65% at 10 dpi compared to the control. The bacterial strain was identified as
Serratia fonticola by metagenomics analysis based on 16s rRNA. Our comparative analysis using KEGG
pathway database and classification of the genes encoding enzymes playing suppressive role on the pathogen
growth will provide new insight for the preparation of effective biopreparate composition and its further
application in vivo assays.