Remote myocardial injury: the protective role of fluoxetine.
Canadian journal of physiology and pharmacology, vol.96, pp.319-327, 2018 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 96
- Publication Date: 2018
- Doi Number: 10.1139/cjpp-2017-0383
- Journal Name: Canadian journal of physiology and pharmacology
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.319-327
- Keywords: fluoxetine, ischemia-reperfusion, infrarenal abdominal aorta, acute cardiac injury, oxidative stress, oxidant/anti-oxidant balance, inflammation, REPERFUSION INJURY, LIPID-PEROXIDATION, ANTIOXIDANT ACTIVITY, EXPRESSION, ISCHEMIA, HEART, ASSAY
- Istanbul University Affiliated: Yes
Abstract
Aortic cross-clamping-induced ischemia-reperfusion (IR) is an important factor in the development of postoperative acute cardiac injury following abdominal aortic surgery. We investigated the possible anti-oxidant/anti-inflammatory effects of fluoxetine (FLX), which is used widely as a preoperative anxiolytic on cardiac injury induced by IR of the infrarenal abdominal aorta. FLX was administered to IR-performed (60 min of ischemia and 120 min of reperfusion) rats for 3 days, once daily at 20 mg/kg i.p. dosage. Results were compared to control and non-FLX-treated IR-performed rats. Serum creatine kinase (CK) and CK-MB levels, lipid hydroperoxide, thiobarbituric acid reactive substances, and pro-oxidant/anti-oxidant balance levels in the IR group were significantly higher whereas superoxide dismutase activity, glutathione, and ferric reducing/anti-oxidant power levels were lower than for the control. IR also increased myeloperoxidase activity, tumor necrosis factor-α, interleukin-1β, and interleukin-6 and decreased interleukin-10 levels. FLX decreased CK, CK-MB, lipid hydroperoxide, thiobarbituric acid reactive substances, and pro-oxidant/anti-oxidant balance levels while increasing superoxide dismutase activity, glutathione, and ferric reducing/anti-oxidant power levels. FLX also decreased myeloperoxidase activity, tumor necrosis factor-α, interleukin-1β, and interleukin-6 levels and increased interleukin-10 levels compared to IR. FLX attenuated the morphological changes associated with cardiac injury. Our study clearly demonstrates that FLX confers protection against aortic IR-induced cardiac injury, tissue leucocyte infiltration, and cellular integrity via its anti-oxidant/anti-inflammatory effects.