The Decreased CD4(+)CD25(+) FoxP3(+) T Cells in Nonstimulated Allergic Rhinitis Patients Sensitized to House Dust Mites


Genc S. , Eroglu H., Kucuksezer U. C. , Aktas-Cetin E. , Gelincik A., Ustyol-Aycan E., ...Daha Fazla

JOURNAL OF ASTHMA, cilt.49, ss.569-574, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 49 Konu: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/02770903.2012.695418
  • Dergi Adı: JOURNAL OF ASTHMA
  • Sayfa Sayıları: ss.569-574

Özet

Objective. Regulatory (CD4(+)CD25(+)) T cells have been shown to play an important role in the development of allergic diseases. This study aims to investigate CD4(+)CD25(+) T cells, Forkhead box P3 (FoxP3(+) cells), and T-helper 1/T-helper 2 (Th1/Th2) cytokines in newly diagnosed allergic rhinitis (AR) patients. Methods. Altogether, 10 subjects with AR and 12 age-matched nonallergic healthy subjects were included in this study. CD4(+)CD25(+) T cells, FoxP3(+) T cells in peripheral blood mononuclear cells (PBMCs) were evaluated by flow cytometry, and the Th1/Th2 cytokine levels were determined by cytometric bead array immunoassay in both PBMC supernatants and nasal lavage fluids. Results. The percentage of CD4(+)CD25(+) T cells were significantly higher, whereas the percentage of FoxP3(+) cells were lower in AR patients compared with healthy subjects. In PBMC culture supernatants, interleukin-10 (IL-10) levels were significantly lower (p = .012), whereas IL-4, IL-5, and tumor necrosis factor-alpha (TNF-alpha) levels in nasal lavage fluids were higher in AR patients compared with healthy subjects (p = .026, p = .015, p = .03, respectively). Conclusions. Our findings indicate that decrease in CD4(+)CD25(+)FoxP3(+) T cell fraction and diminished levels of IL-10 are noteworthy without allergen stimulation in house dust mite AR patients.