Comparative 16s metagenomic analysis of prokaryotic diversity in freshwater and permanent snow-line glacial lakes in Türkiye

Pat F., Fidan Pedük S., Akçay N., Kızıl Pat H. K., Arıcan E.

ARCHIVES OF BIOLOGICAL SCIENCES, cilt.01, sa.01, ss.1-16, 2024 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 01 Sayı: 01
  • Basım Tarihi: 2024
  • Doi Numarası: 10.2298/abs240324016p
  • Dergi Adı: ARCHIVES OF BIOLOGICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.1-16
  • İstanbul Üniversitesi Adresli: Evet

Özet

Freshwater lakes are critical to healthy ecosystems, providing vital services like drinking
water and recreation for surrounding communities. Microorganisms within these ecosystems play
essential roles, driving biogeochemical cycles for elements like carbon, nitrogen, and sulfur. This study
utilized a metagenomic approach to examine the prokaryotic communities of three freshwater lakes in
Türkiye: the Eber and Beyşehir lakes, located at close altitudes (967 m and 1,115 m, respectively),
which serve as primary water sources for nearby communities, and Lake Uludag Buzlu (2,390 m) that
lies at the permanent snow border within the Uludag glacial lake system. Metagenomics allowed us to
identify species, genetic structures, and the functional roles of microorganisms. Employing high-
throughput next-generation sequencing (NGS) technology, we analyzed 16S ribosomal DNA (rDNA)
sequences (V3-V4 regions) from the lake samples. EzBioCloud software facilitated the analysis of
prokaryotic diversity obtained using Illumina NovaSeq technology. While Eber and Beyşehir lakes had
similar diversity, Bacillota dominated in the higher-altitude Lake Uludag Buzlu. Genus-level analysis
revealed Parabacteroides as the most prevalent in Lake Uludag Buzlu, contrasting with Limnohabitans
dominance in Lake Eber; Lake Beyşehir exhibited co-dominance of Limnohabitans and Planktophila.