HLA-A, -B, -C,-DRB1 and-DQB1 allele and haplotype frequencies in Saudis using next generation sequencing technique

Hajeer A. H., AL BALWI M. A., Uyar F. A., ALHAIDAN Y., ALABDULRAHMAN A., AL ABDULKAREEM I., ...More

TISSUE ANTIGENS, vol.82, no.4, pp.252-258, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 82 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.1111/tan.12200
  • Journal Name: TISSUE ANTIGENS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.252-258
  • Keywords: human leukocyte antigen, next generation sequencing, population genetics, Saudi, CLASS-I, RHEUMATOID-ARTHRITIS, HIGH-RESOLUTION, POPULATION, TUNISIANS, LOCI
  • Istanbul University Affiliated: Yes

Abstract

Next generation sequencing (NGS) is a promising technique that can reveal the entire gene sequences and to the highest possible resolution without any phase ambiguities. We have used this technique to investigate the frequencies of HLA-A, -B, -C, -DRB1 and -DQB1 in a Saudi cohort of healthy individuals. We used NGS using the 454 genome sequence (GS) FLX System and Conexio assign atf 454 software to human leukocyte antigen (HLA) genotype eight class I and class II loci. A total of 158 healthy Saudi adults were analyzed. The most frequently observed allele for HLA-A was HLA-A*02:01:01:01 (13.6%); for HLA-B, HLA-B*50:01:01 (15.8%); for HLA-C, HLA-C*06:02:01:01 (18.7%); for HLA-DRB1, HLA-DRB1*07:01:01:01 (26.6%); and for HLA-DQB1, HLA-DQB1*02:01:01 (20.3%). The most common four loci haplotypes in the Saudi population were HLA-A*24:02:01:01-B*08:01:01-C*07:02:01:01-DRB1*03:01:01:01 and HLA-A*23:01:01-B*50:01:01-C*06:02:01:01-DRB1*07:01:01:01.. We have used a highly informative technique for HLA typing of a Saudi healthy cohort to establish allele and haplotype frequencies. These results should prove useful for population studies, disease associations and future planning of the unrelated bone marrow donor registry.