HLA-A, -B, -C,-DRB1 and-DQB1 allele and haplotype frequencies in Saudis using next generation sequencing technique

Hajeer A. H., AL BALWI M. A., Uyar F. A., ALHAIDAN Y., ALABDULRAHMAN A., AL ABDULKAREEM I., ...Daha Fazla

TISSUE ANTIGENS, cilt.82, sa.4, ss.252-258, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 82 Sayı: 4
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1111/tan.12200
  • Dergi Adı: TISSUE ANTIGENS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.252-258
  • Anahtar Kelimeler: human leukocyte antigen, next generation sequencing, population genetics, Saudi, CLASS-I, RHEUMATOID-ARTHRITIS, HIGH-RESOLUTION, POPULATION, TUNISIANS, LOCI
  • İstanbul Üniversitesi Adresli: Evet

Özet

Next generation sequencing (NGS) is a promising technique that can reveal the entire gene sequences and to the highest possible resolution without any phase ambiguities. We have used this technique to investigate the frequencies of HLA-A, -B, -C, -DRB1 and -DQB1 in a Saudi cohort of healthy individuals. We used NGS using the 454 genome sequence (GS) FLX System and Conexio assign atf 454 software to human leukocyte antigen (HLA) genotype eight class I and class II loci. A total of 158 healthy Saudi adults were analyzed. The most frequently observed allele for HLA-A was HLA-A*02:01:01:01 (13.6%); for HLA-B, HLA-B*50:01:01 (15.8%); for HLA-C, HLA-C*06:02:01:01 (18.7%); for HLA-DRB1, HLA-DRB1*07:01:01:01 (26.6%); and for HLA-DQB1, HLA-DQB1*02:01:01 (20.3%). The most common four loci haplotypes in the Saudi population were HLA-A*24:02:01:01-B*08:01:01-C*07:02:01:01-DRB1*03:01:01:01 and HLA-A*23:01:01-B*50:01:01-C*06:02:01:01-DRB1*07:01:01:01.. We have used a highly informative technique for HLA typing of a Saudi healthy cohort to establish allele and haplotype frequencies. These results should prove useful for population studies, disease associations and future planning of the unrelated bone marrow donor registry.