e-Health literacy has become more important in promoting healthy lifestyles in recent years. The purpose of this study was to determine the effect of undergraduate nursing students' e-Health literacy on healthy lifestyle behaviours. This cross-sectional study was conducted during the 2016-2017 academic year at a Nursing Department in the Faculty of Health Sciences in Istanbul. The study population consisted of 232 undergraduate nursing students. The study sample was 205 students who volunteered to participate in the research. The data were collected using the Structured Questionnaire Form, the e-Health Literacy Scale in Adolescent and the Adolescent Lifestyle Profile Scale. The mean score of the e-Health Literacy Scale in Adolescent was 29.50 +/- 5.02. The mean total score of the Adolescent Lifestyle Profile was 112.11 +/- 14.66. There was a positive correlation between students' Internet attitudes and general health-promoting lifestyle (r = 0.273;p < 0.01), indicating the higher the students' e-Health literacy, the higher their levels of healthy lifestyle behaviours. The result showed that e-Health literacy is an important parameter in promoting healthy lifestyle behaviours of nursing students.