Akademik Veri Yönetim Sistemi
6th World Congress on Medicinal and Aromatic Plants, Gazimagusa, Kıbrıs (Kktc), 13 - 17 Kasım 2019, ss.31
The genus Hypericum (Hypericaceae) is represented by 465 species all over the world and 100 taxa
in Turkey(1). Hypericum androsaemum L. has traditionally been used for diuretic, antihepatotoxic,
cholagogue, nephroprotective in Western Europe, and treatment of stomachache and gall bladder
in Turkey. Six extracts; dichloromethane, methanol, water extracts of leaves (HD, HM, HW) and
dichloromethane, methanol, water extracts of mature fruit (FD, FM, FW) were prepared. Chemical
composition of the extracts was identified by LC-MS/MS. HD has hederagenin, FD has herniarin, HM
has (-) epicatechin, FM has (-)epigallocatechin gallate, FW has caffeic acid, and HW has caffeic acid
as major compounds according to the LC-MS/MS analysis. HM has the highest total phenolic content
with 108.51±1.28 µg pyrocatechol equivalent/mg extract, and HD has the highest total flavonoid content
with 86.74±2.29 µg quercetin equivalent/mg extract. HM showed strong antioxidant activity in DPPH
free and ABTS cation radical scavenging assays with IC50:10.94±0.08 and 9.09±05 µg/mL values,
respectively, and better than α-tocopherol used as standard. HM demonstrated significant cupric reducing
antioxidant power (2.576±0.095 absorbance value at 100 µg/mL concentration) and metal chelating
activity (IC50:96.56±1.29 µg/mL). FW showed better activity among the all extract in metal chelating
activity with IC50:77.61±0.79 µg/mL value. FM and HD were more effective than standard cefuroxime
on Staphylococcus epidermidis in antimicrobial assay; their MIC values were 1.22 and 4.88 µg/mL,
respectively. Only HM showed weak antiacetylcholinesterase activity with 21.26±0.82 % ihibition in
all extracts. Same extract showed strong butyrylcholinesterase activity better than galanthamine used as
standard with 87.90±0.50 % ihibition. HD demonstrated the best activity against butyrylcholinesterase
enzyme with 89.13±1.49 % ihibition at 200 µg/mL concentration. HD showed stronger anti-urease
activity than the other extracts with 0.029±0.018 mg/mL IC50 value. FM showed strongest cytotoxic
activity on PC-3 human prostate adenocarcinoma cell line with 7.57 mg/mL IC50 value. As a conclusion,
H. androsaemum can be one of the promising medicinal plants to use for several treatments after further
studies.