Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum beta-lactamases


Vahaboglu H., Ozturk R. , Akbal H., Saribas S. , Tansel O., Coskunkan F.

JOURNAL OF CLINICAL MICROBIOLOGY, vol.36, no.3, pp.827-829, 1998 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 3
  • Publication Date: 1998
  • Doi Number: 10.1128/jcm.36.3.827-829.1998
  • Journal Name: JOURNAL OF CLINICAL MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.827-829

Abstract

A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of bla(OXA) was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.