Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

Demirci, Mehmet; Saribas, Abdulkerim; Ozer, Nigar; Toprak, Sezer; CAGLAR, Emel; ORTAKOYLU, Gonenc; Yuksel, Pelin; Ayaz, Gulsel; Tokman, Hrisi; UYSAL, ÖMER; Dinc, Harika; ZIVER, Tevhide; Kocazeybek, Bekir

doi:10.1016/j.jiph.2018.02.002

Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

Demirci M., Saribas S., Ozer N., Toprak S., CAGLAR E., ORTAKOYLU G., ...More

JOURNAL OF INFECTION AND PUBLIC HEALTH, vol.11, no.5, pp.662-666, 2018 (SCI-Expanded, Scopus)

Publication Type: Article / Article
Volume: 11 Issue: 5
Publication Date: 2018
Doi Number: 10.1016/j.jiph.2018.02.002
Journal Name: JOURNAL OF INFECTION AND PUBLIC HEALTH
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Page Numbers: pp.662-666
Istanbul University Affiliated: Yes

Abstract

Background: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TagMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard.