Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

Demirci, Mehmet; Saribas, Abdulkerim; Ozer, Nigar; Toprak, Sezer; CAGLAR, Emel; ORTAKOYLU, Gonenc; Yuksel, Pelin; Ayaz, Gulsel; Tokman, Hrisi; UYSAL, ÖMER; Dinc, Harika; ZIVER, Tevhide; Kocazeybek, Bekir

doi:10.1016/j.jiph.2018.02.002

Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

Atıf İçin Kopyala

Demirci M., Saribas S., Ozer N., Toprak S., CAGLAR E., ORTAKOYLU G., ...Daha Fazla

JOURNAL OF INFECTION AND PUBLIC HEALTH, cilt.11, sa.5, ss.662-666, 2018 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 11 Sayı: 5
Basım Tarihi: 2018
Doi Numarası: 10.1016/j.jiph.2018.02.002
Dergi Adı: JOURNAL OF INFECTION AND PUBLIC HEALTH
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.662-666
İstanbul Üniversitesi Adresli: Evet

Özet

Background: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TagMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard.