Vitamin D mitigates atherosclerosis risk in TNF-alpha-induced endothelial cells

Onal B., ALAYLIOĞLU M., DURSUN E., GEZEN AK D.

TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, vol.21, no.4, pp.701-706, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 4
  • Publication Date: 2022
  • Doi Number: 10.4314/tjpr.v21i4.3
  • Journal Name: TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, International Pharmaceutical Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Page Numbers: pp.701-706
  • Keywords: Atherosclerosis, Cytokines, Vitamin D, Inflammation, TNF-alpha, INFLAMMATORY CYTOKINES, CARDIOVASCULAR-DISEASE, D SUPPLEMENTATION, D DEFICIENCY, PREVENTION, TRIAL
  • Istanbul University Affiliated: No

Abstract

Purpose: To determine the effect of vitamin D on endothelial function in a cell line model of inflammation. Methods: Human saphenous vein cells were treated with 1,25-dihydroxyvitamin D3 (1,25-OHD) and/or TNF-??, and the expression levels of 20 different atherosclerotic cytokines were examined in the treated cells using a human atherosclerosis antibody array. The STRING and PANTHER analyses were also performed to determine the possible pathway interactions of these four pro-atherosclerotic and two antiResults: The expression levels of pro-atherosclerotic cytokines GM-CSF, CCL20, CCL5, and TNF-?? decreased upon 1,25-OHD administration, whereas those of anti-atherosclerotic cytokines PDGFB and Conclusion: Vitamin D could mitigate cardiovascular risks by mediating the expression of inflammatory cytokines; however, this needs to be verified via in vivo studies.