Akademik Veri Yönetim Sistemi
ULTRASTRUCTURAL PATHOLOGY, cilt.45, sa.6, ss.384-390, 2021 (SCI-Expanded)
Monobenzyl ether of hydroquinone (MBEH) is a topical depigmentation agent used by vitiligo patients to even the skin tone. We aimed to investigate the effects of MBEH on 3T3 mouse fibroblasts. Fibroblasts were treated with 250 mu M, 500 mu M, and 750 mu M MBEH and vehicle (EtOH:DMSO) for 24 hours. Cell numbers of 250 mu M, 500 mu M, and 750 mu M MBEH treated and vehicle groups decreased significantly compared to control group. TUNEL positive cell rate increased with MBEH concentration. In electron microscopic examination, control and vehicle groups showed active cells features, while mitochondrial swelling and cristae loss were seen in 250 mu M MBEH-treated group. In cytoplasm of 500 mu M MBEH-treated group, there were many multivesicular bodies and autophagic vacuoles. As an indication of apoptosis, cell membrane blebs and reduction in cell size were observed. In 750 mu M MBEH-treated group, cells were completely degenerated. Our findings show that MBEH, which is used as a depigmentation agent to lighten the skin by destroying melanocytes, may also have dose-dependent negative effects on the viability of 3T3 mouse fibroblasts, and these may be mediated through autophagic and apoptotic cell death mechanisms.