Phenolic Profiles, Antimicrobial and Cytotoxic Properties of Both Micropropagated and Naturally Growing Plantlets of Calamintha sylvatica subsp. sylvatica Bromf.

CÜCE M., BEKİRCAN T., Laghari A. H., Sokmen M., Sokmen A., Ucar E., ...Daha Fazla

NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, cilt.47, sa.4, ss.1145-1152, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47 Sayı: 4
  • Basım Tarihi: 2019
  • Doi Numarası: 10.15835/nbha47411474
  • Dergi Adı: NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1145-1152
  • Anahtar Kelimeler: antimicrobial activity, Calamintha sylvatica, cytotoxic activity, micropropagation, rosmarinic acid, HAIRY ROOT CULTURES, ROSMARINIC ACID, ESSENTIAL OILS, ANTIOXIDANT PROPERTIES, SUSPENSION-CULTURES, SOLVENT-EXTRACTION, LAMIACEAE, GROWTH, PROLIFERATION, L.
  • İstanbul Üniversitesi Adresli: Evet

Özet

A rapid micropropagation protocol was designed to produce Calamintha sylvatica plantlets by using nodal segments as explants for the shoot formation. 6-BA favored the highest shoot formation and biomass yield, whilst kinetin was found superior for the highest shoot length (38.97 +/- 2.85 mm) and node numbers (2.89 +/- 0.63). Rosmarinic acid was detected as major phenolic acid, ranging from 7.59 mg/100 g to 81.44 mg/100 g. Hexane extracts from natural and in vitro propagated plantlets showed activity only against Staphylococcus aureus ATCC 25923 with MIC values at 6.25 and 3.33 m/mL, respectively while in the latter case, extracts from natural plantlets exerted higher cytotoxic activity than those of micropropagated ones (IC50 values were 83 mu g/mL and 98 mu g/mL on HeLa cells, respectively). C. sylvatica showed high micropropagation performance and produced remarkable amount of rosmarinic acid in vitro as well as antimicrobial and cytotoxic effect.