Characterization of biodegradable chitosan microspheres containing vancomycin and treatment of experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus with prepared microspheres

Cevher E., Orhan Z., Mulazimoglu L., Sensoy D., Alper M., Yildiz A., ...More

INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol.317, pp.127-135, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 317
  • Publication Date: 2006
  • Doi Number: 10.1016/j.ijpharm.2006.03.014
  • Journal Name: INTERNATIONAL JOURNAL OF PHARMACEUTICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.127-135
  • Keywords: vancomycin, biodegradable microspheres, chitosan, spray drying method, methicillin-resistant Staphylococcus aureus, experimental osteomyelitis, IMPLANT-RELATED OSTEOMYELITIS, IN-VITRO, DELIVERY-SYSTEM, TREATING OSTEOMYELITIS, BIOABSORBABLE POLYMER, PECTIN MICROSPHERES, TOPICAL VANCOMYCIN, MRSA OSTEOMYELITIS, ANTIBIOTIC RELEASE, PLGA MICROSPHERES
  • Istanbul University Affiliated: Yes

Abstract

The biodegradable chitosan microspheres containing vancomycin hydrochloride (VANCO) were prepared by spray drying method with different polymer:drug ratios ( 1: 1, 2:1, 3:1 and 4: 1). Thermal behaviour, particle size and distribution, morphological characteristics, drug content, encapsulation efficiency, in vitro release assessments of formulations have been carried out to obtain suitable formulation which shows sustained-release effect when implanted. Sterilized VANCO loaded microspheres were implanted to proximal tibia of rats with methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis. Intramuscular (IM) injection of VANCO for 21 days was applied to another group for comparison. After 3 weeks of treatment, bone samples were analysed with a microbiological assay.