Determination of amlodipine in human plasma by high-performance liquid chromatography with fluorescence detection

Tatar S., Atmaca S.

JOURNAL OF CHROMATOGRAPHY B, vol.758, no.2, pp.305-310, 2001 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 758 Issue: 2
  • Publication Date: 2001
  • Doi Number: 10.1016/s0378-4347(01)00197-9
  • Journal Name: JOURNAL OF CHROMATOGRAPHY B
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.305-310
  • Istanbul University Affiliated: Yes

Abstract

A sensitive and specific HPLC method has been developed for the assay of amlodipine in human plasma. The assay involves derivatization with 4-chloro-7-nitrobenzofurazan (NBD-Cl), solid-phase extraction on a silica column and isocratic reversed-phase chromatography with fluorescence detection. Nortriptyline hydrochloride was used as an internal standard. The assay was linear over the concentration range of 0.25-18.00 ng/ml. Both of the within-day and day-to-day reproducibility and accuracy were less than 11.80% and 12.00%, respectively. The plasma profile following a single administration of 10 mg amlodipine to a healthy volunteer was presented. (C) 2001 Elsevier Science B.V. All rights reserved.