Determination of amlodipine in human plasma by high-performance liquid chromatography with fluorescence detection

Tatar S., Atmaca S.

JOURNAL OF CHROMATOGRAPHY B, cilt.758, sa.2, ss.305-310, 2001 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 758 Sayı: 2
  • Basım Tarihi: 2001
  • Doi Numarası: 10.1016/s0378-4347(01)00197-9
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY B
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.305-310
  • İstanbul Üniversitesi Adresli: Evet

Özet

A sensitive and specific HPLC method has been developed for the assay of amlodipine in human plasma. The assay involves derivatization with 4-chloro-7-nitrobenzofurazan (NBD-Cl), solid-phase extraction on a silica column and isocratic reversed-phase chromatography with fluorescence detection. Nortriptyline hydrochloride was used as an internal standard. The assay was linear over the concentration range of 0.25-18.00 ng/ml. Both of the within-day and day-to-day reproducibility and accuracy were less than 11.80% and 12.00%, respectively. The plasma profile following a single administration of 10 mg amlodipine to a healthy volunteer was presented. (C) 2001 Elsevier Science B.V. All rights reserved.