Does copper stress lead to spindle misposition-dependent cell cycle arrest?

Tarhan C., Sarikaya A. T.

GENETICS AND MOLECULAR RESEARCH, cilt.11, sa.4, ss.3824-3834, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.4238/2012.october.25.1
  • Dergi Adı: GENETICS AND MOLECULAR RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3824-3834
  • Anahtar Kelimeler: Schizosaccharomyces pombe, Copper stress, Cell cycle, GENE-EXPRESSION, FISSION YEAST, SACCHAROMYCES-CEREVISIAE, DIFFERENTIAL DISPLAY, MICROARRAY ANALYSIS, PLASMA-MEMBRANE, BINDING PROTEIN, SWIRM DOMAIN, MITOTIC EXIT, MECHANISMS
  • İstanbul Üniversitesi Adresli: Evet

Özet

Because of its specific electrochemical properties, copper is an essential heavy metal for living organisms. As with other heavy metals, high levels can provoke damage. We examined gene expression under copper stress in wild-type fission yeast (Schizosaccharomyces pombe) through differential display. After the EC50 concentration of CuSO4 was determined as 50 mu M, total RNA was isolated from cells treated or not with copper. The expression level of SPCC1682.13, ppk 1, SPBC2F12.05c, and adg2 genes increased significantly under copper stress. Considering the functions of these genes are related to the cell cycle, cell division and chromosome dynamics, we hypothesize that retardation of the cell cycle under copper stress is relevant to the events that depend on the functions of these genes.