Ameliorative Effects of Apocynin on Histopathological and Biochemical Changes Induced by Chloroquine and Gamma Radiation in Testes Experimental Model

Köroğlu P., Ertik O., Sezen Us A., Us H., Çöremen M., Bulan N. Ö., ...Daha Fazla

26. Ulusal Elektron Mikroskopi Kongresi, Eskişehir, Türkiye, 20 - 23 Eylül 2023, ss.104

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Eskişehir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.104
  • İstanbul Üniversitesi Adresli: Evet

Özet

Gamma radiation can pass completely through the human body; as they pass through, they can cause ionizations that damage tissue and DNA. Gamma radiation, an ionising radiation, impairs the reproductive potential. Chloroquine is a class of antimalarial drugs currently used for the treatment of various diseases including cancer and viral infections and is a late autophagy inhibitor. It is known that apocynin has a vital role in reducing oxidative stress. We aim to evaluate the damage caused by radiation and chloroquine on testicular tissue and determine the effects of apocynin administration on male reproductive capacity as a protective factor to reduce or prevent damage histopathologically and biochemically. Male rats were divided into 8 groups. Control, chloroquine, apocynin, radiation, chloroquine+apocynin, chloroquine+radiation, apocynin+radiation, chloroquine+apocynin+radiation. The rats were sacrificed, testicular tissues were collected for histological and biochemical examinations. Testicular tissues total antioxidant status (TAS), total oxidant status (TOS), reactive oxygen species (ROS) and oxidative stress index (OSI) levels were assessed. The activities of acid phosphatase (ACP) and alkaline phosphatase (ALP) were respectively evaluated by the methods of testicular tissues. In the radiation and chloroquine groups, enlargement of the intercellular spaces, degenerative and atrophic tubules and morphological other damage parameters were observed in the testis tissues. Histological findings were reversed in the apocynin treated groups. Biochemical parameters revealed that damage occurred in the groups given chloroquine and radiation. The damages were reversed or attenuated by the administration of apocynin to the groups. Both radiation and chloroquine caused deterioration in testicular histology and increased oxidant damage. It was concluded that apocynin plays a protective/therapeutic role by reducing histological and biochemical damage, as well as regulates oxidant/antioxidant balance in testicular tissue.