Oxidative protein damage parameters in plasma in chronic experimental diabetes in rats


Kayali R., Telci A., Cakatay U., Karaca C., Akcay T., Sivas A., ...More

EUROPEAN JOURNAL OF MEDICAL RESEARCH, vol.8, no.7, pp.307-312, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 7
  • Publication Date: 2003
  • Journal Name: EUROPEAN JOURNAL OF MEDICAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.307-312
  • Istanbul University Affiliated: Yes

Abstract

An increase in oxidative stress may contribute to the development of oxidative protein damage (OPD) in the streptozotocin-diabetic rat. To show the effect of hyperglycemia in promoting OPD, we determined protein carbonyl (PCO), nitrotyrosine (NT), total thiol (T-SH) and advanced oxidation protein product (AOPP) levels as markers of OPD, and lipid hydroperoxide (LHP) levels as a marker of lipid peroxidation in plasma of acute and chronic diabetic male Sprague-Dawley rats and their controls. The levels of the studied markers, except NT, were determined by colorimetric methods. NT levels were measured by ELISA.