Maedi-visna virus in Turkish sheep: a preliminary serological survey using ELISA tests


PREZIUSO S., Or M. E., GIAMMARIOLI M., Kayar A., FELIZIANI F., Gonul R., ...More

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, vol.34, no.3, pp.289-293, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 3
  • Publication Date: 2010
  • Doi Number: 10.3906/vet-0905-35
  • Journal Name: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.289-293
  • Istanbul University Affiliated: Yes

Abstract

The maedi-visna virus (MVV) is distributed worldwide and, to date, no epidemiological surveys have been carried out using ELISA testing in Turkey. The aims of this study were as follows: i) to verify the diagnostic performance of a home-made ELISA test (HM-ELISA) using Turkish ovine sera in comparison with other commercially available ELISA tests, and ii) to perform a preliminary epidemiological survey in Istanbul province. The MVV seroprevalence in Istanbul province was 15.3%. Older sheep were 5 times more likely to be positive than younger animals, ewes were 3 times more likely to be positive than rams, and all the Red Karaman and White Karaman sheep tested were seronegative. The performance of the HM-ELISA and that of the other commercial tests using Turkish ovine serum samples was similar; therefore, the HM-ELISA test is suitable for use in extensive epidemiological surveys and eradication programs in Turkey.