COORDINATED IMMUNOMODULATORY AND PROLIFERATIVE EFFECTS OF A POLYPHENOL-BROMELAIN COMBINATION IN J774 MACROPHAGES


Elgun T., Musteri Oltulu Y., Arslan H. I., Gundogan G. I., Ciraci E., Nazligul E., ...Daha Fazla

Comptes Rendus de L'Academie Bulgare des Sciences, cilt.79, sa.3, 2026 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 79 Sayı: 3
  • Basım Tarihi: 2026
  • Doi Numarası: 10.7546/crabs.2026.03.10
  • Dergi Adı: Comptes Rendus de L'Academie Bulgare des Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, MathSciNet, zbMATH
  • Anahtar Kelimeler: bromelain, cell cycle, IL-10, IL-6, macrophage, polyphenol, total antioxidant capacity
  • İstanbul Üniversitesi Adresli: Evet

Özet

Polyphenols are a diverse family of plant secondary metabolites with well-documented antioxidant and anti-inflammatory activities. Bromelain is a pineapple-derived cysteine protease with documented anti-inflammatory activity. This study aimed to comprehensively evaluate the immunomodulatory, anti-inflammatory, and proliferative effects of a plant-derived polyphenol–bromelain (PB) combination (SammyVit, Turkiye) on the murine macrophage cell line J774. Cells were cultured in RPMI-1640 medium and treated with PB at 1%, 2.5%, 5%, 10%, and 20%. Inflammatory responses were assessed by measuring interleukin-6 (IL-6) and interleukin-10 (IL-10) levels; oxidative balance was evaluated using total antioxidant capacity (TAC). Cell-cycle dynamics were analyzed by flow cytometry. PB significantly reduced IL-6, increased IL-10 and TAC, and elevated the proportion of cells in the S and G2/M phases, supporting enhanced proliferative activity. Collectively, the results indicate coordinated anti-inflammatory, antioxidant, and pro-proliferative actions consistent with suppression of redox-sensitive pathways and reinforcement of a repair-compatible macrophage phenotype. The data support further mechanistic dissection and motivate translational testing in primary human macrophages and relevant in vivo models.