CLASS B TRICHOTHECENE CHEMOTYPING IN Fusarium SPECIES BY PCR ASSAY


Yoruk E., Gazdagli A., Albayrak G.

Genetika-Belgrade, vol.46, no.3, pp.661-669, 2014 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 46 Issue: 3
  • Publication Date: 2014
  • Doi Number: 10.2298/gensr1403661y
  • Title of Journal : Genetika-Belgrade
  • Page Numbers: pp.661-669

Abstract

Fusarium isolates are divided into three chemotypes according to produce of
class B trichothecenes; 3-acetyldeoxynivalenol (3ADON) 15-acetyldeoxynivalenol
(15ADON) and nivalenol (NIV) and 4- acetyldeoxynivalenol (NIV) chemotypes. In this
study, chemotyping of seventeen isolates from Turkey and Iran belonging to F.
graminearum, F. culmorum, F. poae and F. pseudograminearum species were carried
out by polymerase chain reaction (PCR) analysis. While all F. culmorum and F. poae
isolates determined as 3ADON, remaining F. graminearum and F. pseudograminearum
isolates were either 3ADON or 15ADON chemotypes. A common band of 583 bp long
DNA fragment was amplified in all of F. culmorum and F. poae, one F.
pseudograminearum (21F) and four F. graminearum (14F, sh14, sh15, sh7) isolates
with 3ADON chemotype. However, remaining two F. pseudograminearum and four F.
graminearum isolates with 15ADON chemotype, yielded amplicons that of 863 bp. It
was shown that 3ADON was more predominant chemotype from other class B
trichothecenes. This is the first report on chemotyping of F. poae and F.
pseudograminearum isolates and also to show presence of 3ADON chemotype in F.
graminearum isolate from Turkey.

Fusarium isolates are divided into three chemotypes according to produce of class B trichothecenes; 3-acetyldeoxynivalenol (3ADON) 15-acetyldeoxynivalenol (15ADON) and nivalenol (NIV) and 4-acetyldeoxynivalenol (NIV) chemotypes. In this study, chemotyping of seventeen isolates from Turkey and Iran belonging to F. graminearum, F. culmorum, F. poae and F. pseudograminearum species were carried out by polymerase chain reaction (PCR) analysis. While all F. culmorum and F. poae isolates determined as 3ADON, remaining F. graminearum and F. pseudograminearum isolates were either 3ADON or 15ADON chemotypes. A common band of 583 bp long DNA fragment was amplified in all of F. culmorum and F. poae, one F. pseudograminearum (21F) and four F. graminearum (14F, sh14, sh15, sh7) isolates with 3ADON chemotype. However, remaining two F. pseudograminearum and four F. graminearum isolates with 15ADON chemotype, yielded amplicons that of 863 bp. It was shown that 3ADON was more predominant chemotype from other class B trichothecenes. This is the first report on chemotyping of F. poae and F. pseudograminearum isolates and also to show presence of 3ADON chemotype in F. graminearum isolate from Turkey.