In vitro effects of peroxynitrite on human spermatozoa

OZTEZCAN S., Turkoglu U. , KERVANCIOGLU E. , Kocak T. , Kocak-Toker N. , AYKAC-TOKER G.

ANDROLOGIA, vol.31, no.4, pp.195-198, 1999 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 4
  • Publication Date: 1999
  • Doi Number: 10.1046/j.1439-0272.1999.00279.x
  • Title of Journal : ANDROLOGIA
  • Page Numbers: pp.195-198


In the present study, the in vitro effects of peroxynitrite on sperm motility, lipid peroxidation and sulfhydryl content were examined. Sperm percentage motility and movement characteristics were assessed by a computer-assisted system. Lipid peroxidation was measured by determining malondialdehyde levels using the thiobarbituric acid (TBA) method. Sperm sulfhydryl content was measured by a spectrophotometric method based on reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) by sulfhydryl groups. Percentage motility, movement characteristics and sulfhydryl content decreased significantly in peroxynitrite-treated samples compared to decomposed peroxynitrite-treated samples. Lipid peroxidation in peroxynitrite-treated samples was significantly higher than in decomposed peroxynitrite-treated samples. These results indicate that peroxynitrite anion may cause sperm dysfunction through lipid peroxidation stimulation and total SH depletion.