In this study, aerial parts of Urtica dioica L. have been assesed for antioxidant activity and antimicrobial activity. Antioxidant activity was done according to the liposome TBA method. Antimicrobial activity was done according to the serial dilution method. The ethanol extract of the aerial parts of U. dioica showed considerable antioxidant activity by inhibition of lipid peroxidation (IC50 0.83 mg/mL). The dichlormethane extract showed weak antimicrobial activity (MIC > 50 μg/mL), but slightly better activity against the yeast Candida albicans (MIC 40 μg/mL). In contrast to some previous reports, the water, methanol and ethanol extracts displayed negligible antimicrobial effects.