In this study, we aimed an increase in Multiple pregnancy and a decrease in MDA (Malondialdehyde) levels in Tuj breed sheep which are in non-breeding season by administration of testosterone antibody, beta-carotene and vitamin E. Testosterone antibody was obtained from a castrated Tuj ram following injections of testosterone. Experiment was carried Out in 29 sheep divided into three groups. Group I (n=10) was administered testosterone antibody, beta-carotene and vitamin E one week prior to placement of vaginal sponges. Group II (n=9) was administered testosterone antibody alone and Group III (n=10) was control group. Oestrus rates were detected as 90%, 88.9% and 100% in Groups I,II and III, respectively. Pregnancy rates were found 40%, 33.3% and 50% in Groups I,II and III, respectively. Multiple pregnancy was seen in Groups I and II but not in control group. MDA levels were increased in all sheep regardless of pregnancy on day 28 after vaginal sponge application, but no significant difference was detected between the gropus((P > 0.05). It was concluded that administration of testosterone antibody and beta-carotene caused an increase in multiple pregnancy rate in sheep.