Comparison of the antibiofilm activity of plant-derived compounds furanone C30 and ellagic acid C11 with antibiotics against Pseudomonas aeruginosa
İstanbul Kuzey Klinikleri, vol.12, no.1, pp.21-28, 2025 (ESCI, Scopus, TRDizin)
- Publication Type: Article / Article
- Volume: 12 Issue: 1
- Publication Date: 2025
- Doi Number: 10.14744/nci.2023.89757
- Journal Name: İstanbul Kuzey Klinikleri
- Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, Central & Eastern European Academic Source (CEEAS), Directory of Open Access Journals, TR DİZİN (ULAKBİM)
- Page Numbers: pp.21-28
- Istanbul University Affiliated: No
Abstract
OBJECTIVE: Pseudomonas aeruginosa is considered one of the leading pathogens responsible for community- and health- care-associated infections. P. aeruginosa infections are difficult to treat due to antibiotic resistance, various virulence factors, and the capacity to build biofilms, which provide resistance to drugs and immune cells. Antibiotics used to treat biofilm-asso- ciated infections over an extended period resulted in the establishment of resistance strains. In this study, we comparatively investigated the efficacy of natural compounds (Furanone-C30 [F], Ellagic-acid C11 [EA], Tobramycin [TOB], Ciprofloxacin [CIP], and Meropenem [MEM]) and antibiotics in inhibiting and eradicating P. aeruginosa biofilm. METHODS: The Minimum Biofilm Inhibition Concentrations (MBIC) and Minimum Biofilm Eradication Concentrations (MBEC) were determined using a micro broth dilution technique, and their effects on the biofilm were evaluated by crystal violet staining and cell viability tests (MTT). RESULTS: F significantly suppressed P. aeruginosa biofilm formation in a dose-dependent way, with 100% inhibition at 512 and 256 g/mL and 92% inhibition at 128 μg/mL. F also eradicated 92.9% of the biofilm at 512 μg/mL and 90% at 256 μg/ mL. EA provided 41.6% biofilm inhibition and 33.1% biofilm eradication at 512 μg/mL. CONCLUSION: Our findings indicate that the natural compounds displayed a dose-dependent effect on P. aeruginosa biofilm, with F being found to be more effective than EA. In conclusion, this study suggests that furanone may hold promise as a natu- ral alternative for the treatment of P. aeruginosa biofilm and highlights the need for further research to support this hypothesis.