Evaluation of Chromogenic Factor VIII Assay Compared with One-Stage Clotting Assay

Akkaya E., Hatiboglu S., Koc B., Genc S., Unuvar A., Karaman S., ...Daha Fazla

CLINICAL LABORATORY, cilt.66, sa.10, ss.1937-1943, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66 Sayı: 10
  • Basım Tarihi: 2020
  • Doi Numarası: 10.7754/clin.lab.2020.191145
  • Dergi Adı: CLINICAL LABORATORY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.1937-1943
  • Anahtar Kelimeler: plasma FVIII activity, hemophilia A, one-stage assay, chromogenic assay, method comparison, MILD HEMOPHILIA-A, FVIII-C ASSAY, DISCREPANCY, MUTATIONS, THROMBIN, STANDARDIZATION, INACTIVATION, COAGULATION, VALIDATION, PHENOTYPE
  • İstanbul Üniversitesi Adresli: Evet

Özet

Background: Congenital factor VIII (FVIII) deficiency causes hemophilia A due to different types of defects in the FVIII gene. Although the chromogenic measurement is the reference method and shows less variability, a one-stage assay is the most commonly preferred method for measurement of FVIII. In this study, we aimed to evaluate the analytical performances of chromogenic and one-stage assays, and compare the results prior to introduction of newly developed extended half-life recombinant FVIII products.