Hemodialysis machines are the devices with risk of infection. Disinfection and sterilization are the main parts of the injection control in the hemodialysis units. The objective of the disinfection of the hemodialysis system is to kill all microorganisms or to decrease the number of them. All disinfection process performed in the hemodialysis units are traced by the Health Ministry. So that, it is planned to determine the conformities and to take the required preventions without harming to the patient. During hemodialysis system disinfection, not only hemodialysis machine but also water treatment system and distribution system should be disinfected. Because they require a comprehensive study, the responsibility has been given to the biomedical staff rather than the user. Therefore, it is prerequisite that a biomedical staff must know the disinfection of the hemodialysis system. In this study, the information about the disinfection of the hemodialysis systems and the related instructions are given. And, the disinfection tracking system created by the Biomedical Departmant of Istanbul University Hospitals is presented.