O-6-methylguanine DNA methyltransferase activity in diabetic patients

Akcay T. , Dincer Y. , Celebi N., Ilkova H.

DIABETES RESEARCH AND CLINICAL PRACTICE, vol.61, no.1, pp.1-6, 2003 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 61 Issue: 1
  • Publication Date: 2003
  • Doi Number: 10.1016/s0168-8227(03)00063-9
  • Page Numbers: pp.1-6


In the present study, we evaluated O-6-methylguanine-DNA methyltransferase (MGMT) activity in diabetic patients. The study was performed on 27 patients with Type 1 diabetes, and 42 with Type 2 diabetes. Patients with complications were excluded from the study. 36 non-diabetic volunteers, non-smokers who do not consume alcoholic beverage, were chosen from the medical staff as control subjects. MGMT activity was measured by the transfer of radiolabeled methyl groups from a prepared methylguanine-DNA substrate to the enzyme fraction of leukocyte extract. Leukocyte MGMT activity was significantly reduced in both Type 1 and Type 2 diabetes patients as compared with control subjects (P < 0.001). The present study demonstrates decreased MGMT activity in leukocytes from patients with Type 1 and Type 2 diabetes. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.