The effects of various BSA levels in different media on development in in vitro culture of mouse embryos


Evecen M., Pabuccuoglu S., Alkan S., Ileri K.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.28, sa.2, ss.337-342, 2004 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 2
  • Basım Tarihi: 2004
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.337-342
  • İstanbul Üniversitesi Adresli: Evet

Özet

Various BSA (bovine serum albumin) levels and media effects on development of 2-cell stage mouse embryos in in vitro culture were investigated in this 2 stage study. In the first stage, 1400 2-celled mouse embryos were cultured in 2 various BSA-bearing culture media for 96 h and directly observed microscopically. In the second stage, 1024 2-celled mouse embryos cultured for the same period and cell numbers (nuclei of cells) were determined by staining techniques.

Various BSA (bovine serum albumin) levels and media effects on development of 2-cell stage mouse embryos in in vitro culture were investigated in this 2 stage study. In the first stage, 1400 2-celled mouse embryos were cultured in 2 various BSA bearing culture media for 96 h and directly observed microscopically. In the second stage, 1024 2-celled mouse embryos cultured for the same period and cell numbers (nuclei of cells) were determined by staining techniques. Embryos were cultured in both stages in M 16 and WhittenÕs media containing 0 (control), 0.3, 1, 3, 9, 18 and 36 mg/ml of BSA. The highest blastocyst rate was 94.57% ± 7.43, observed in 3 mg/ml BSA-containing WhittenÕs medium group (P < 0.05). The highest hatching + hatched blastocyst rates were obtained in the same group with 67.70% ± 25.22. The highest nucleus number in the M 16 medium was in the 1 mg/ml BSA group (95.45 ± 9.61). In the M16 medium group, the 3 mg/ml BSA group showed the highest development rate in the first stage of the study; however, the nucleus numbers in this group (81.37 ± 18.31), were significantly lower than the nucleus numbers of the 1 mg/ml BSA group (P < 0.05). It was concluded that the optimal benefit to in vitro culture of 2-cell mouse embryos could be maintained when BSA was used as a protein source at 1 mg/ml and 3 mg/ml doses, and that M16 or WhittenÕs media could be used successfully as a culture medium. Developmental evaluations of in vitro cultured mouse