Digging into the extracellular matrix of a complex microbial community using a combined metagenomic and metaproteomic approach

Albertsen M., Stensballe A., Nielsen K. L., Nielsen P. H.

WATER SCIENCE AND TECHNOLOGY, cilt.67, sa.7, ss.1650-1656, 2013 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67 Sayı: 7
  • Basım Tarihi: 2013
  • Doi Numarası: 10.2166/wst.2013.030
  • Dergi Adı: WATER SCIENCE AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.1650-1656
  • Anahtar Kelimeler: activated sludge, EBPR, exopolymers, extracellular proteins, metagenomics, metaproteomics, FILAMENTOUS BACTERIA, SLUDGE, PROTEIN, EXTRACTION, ADHESINS, ABUNDANT
  • İstanbul Üniversitesi Adresli: Hayır

Özet

Knowledge about identity and function of extracellular polymeric substances (EPS) in complex microbial communities is sparse, although these components have a large influence on the function of the microbial communities. We investigated the presence of selected genes potentially involved in EPS production in a 145 Mbp metagenome prepared by Illumina sequencing from a full-scale wastewater treatment plant carrying out enhanced biological phosphorus removal (EBPR). A range of genes involved in alginate production was identified and assigned mainly to bacteria from the phylum Bacteroidetes. Furthermore, several proteins in the EPS matrix were extracted, purified and identified by mass spectrometry. By using the metagenome as a reference for the metaproteomic analysis, more proteins were identified compared to using only publicly available databases. This illustrates the low degree of similarity between the bacteria in the EBPR community and the sequenced bacteria in the public databases. Hence, the combination of metagenomics and metaproteomics presented here is needed to investigate the identity of the proteins in the EPS matrix.