Isolation of an Arabidopsis protein binds to LexA operator site in yeast

Gurel F.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.16, sa.2, ss.109-112, 2002 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 2
  • Basım Tarihi: 2002
  • Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.109-112
  • İstanbul Üniversitesi Adresli: Hayır

Özet

We have isolated an Arabidopsis protein designated 1-2 Ara, which activates transcription from a LexA-specific promoter in yeast. The 1-2 Ara protein specifically binds to the LexA operator sites and activates expression of the lacZ and HIS3+ reporter genes. 1-2 Ara binding to the LexA operators was specific because 1-2 Ara did not recognize the Gal4-inducible promoter. To understand whether 1-2 Ara has functions similiar to the bacterial LexA protein, we use an indicator E. coli strain which allows to specifically assay the LexA-mediated repression activity. Studies on molecular cloning and expression of 1-2 Ara for further analyses are in progress.