Antiproliferative effects of fluorine substitute 3,5-di-tert-butylphenol bearing Schiff bases using CFSE-based cell proliferation assay
CURRENT SCIENCE, vol.112, no.3, pp.619-624, 2017 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 112 Issue: 3
- Publication Date: 2017
- Doi Number: 10.18520/cs/v112/i03/619-624
- Journal Name: CURRENT SCIENCE
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.619-624
- Istanbul University Affiliated: Yes
Abstract
The determination of antiproliferative properties of compounds on tumour cells is important for assessment of their efficacy in cancer treatment. CFSE-labelled K562 cells were incubated with doxorubicin and ortho-or para-fluorosubstitute Schiff bases (compounds 1 and 2 respectively). CFSE intensities were analysed using flow cytometry. K562 cells treated with doxorubicin resulted in homogeneous high intensity fluorescence after 96 h of incubation. Schiff bases exhibited antiproliferative effects, but lower than doxorubicin. Our results reveal that CFSE assay can be used for determining in vitro antiproliferative features of anticancer drugs and/or compounds from herbal or chemical sources.