Concanavalin A and Phytohaemagglutinin Stimulated Lymphoproliferative Responses in Cord Blood Mononuclear Cells

Suzergoz F., Kiran B., DENİZ G., Evcimik M. F., Cen O.

TURKISH JOURNAL OF IMMUNOLOGY, vol.10, no.1, pp.13-18, 2005 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 1
  • Publication Date: 2005
  • Journal Name: TURKISH JOURNAL OF IMMUNOLOGY
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
  • Page Numbers: pp.13-18
  • Keywords: Cord blood, Con A, PHA, Lymphoproliferation, GVHD., VERSUS-HOST-DISEASE, T-LYMPHOCYTES, GRAFT, ACTIVATION, UNRESPONSIVENESS, TRANSPLANTATION, CHILDREN
  • Istanbul University Affiliated: Yes

Abstract

Experimental and clinical evidences have demonstrated that umbilical cord blood is an efficient source of transplantable haematopoietic stem and progenitor cells. After transplantation, initial exposure to alloantigen-presenting cells (allo-APC) induced lymphoproliferative responses in cord blood T cells. However, this response is lower than that by adult T cells. The aim of this study was to investigate and compare lymphoprolifertive responses of cord blood mononuclear cells (CBMC) and adult peripheral blood mononuclear cells (PBMC). CBMC and PBMC immunoproliferative responses were measured by MTT assay after stimulation of respective primary culture cells with phytohaemagglutinin (PHA) or Concanavalin A (Con A) for 76 hours. Lymphoproliferative response of CBMC was significantly lower compared to that of PBMC.