Sorting of micron-sized particles using holographic optical Raman tweezers in aqueous medium

Parlatan U., Basar G., Başar G.

JOURNAL OF MODERN OPTICS, vol.66, no.2, pp.228-234, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 66 Issue: 2
  • Publication Date: 2019
  • Doi Number: 10.1080/09500340.2018.1515378
  • Journal Name: JOURNAL OF MODERN OPTICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.228-234
  • Istanbul University Affiliated: Yes

Abstract

We have constructed a holographic optical tweezers system combined with Raman spectroscopy to sort trapped particles. Our software automatically moves the trapped objects to the measurement positions to obtain individual Raman signals from multiple trapped particles. We performed the sorting by comparing their spectra with the previously measured training dataset using the correlation coefficients. We used yeast cells and polystyrene beads as test particles. This study aims to show that biological particles can be separated using single cell analysis with combined holographic optical tweezers and Raman spectroscopy system.