In our study, we developed a new method for fluorescence probes which is novel, inexpensive and renders useful information for prognosis on tumor tissues. It could be used in place of the receptors analysis. Comparative studies applying fluorescence probe techniques to the determination of Intracytoplasmic Immunoglobulin (Ic-IgG) have shown to generally provide more precise results and to be time saving as compared to receptors and DNA analysis. The use of fluorescence probe techniques for the measuring of Intra cytoplasmic Immunoglobulin (Ic-IgG) is well established for breast tumors. Ic-IgG has been determined in mammary turner cells with fluorescein isothiocynate (FTIC) labeled anti human IgG using the flow cytometer. Ic-IgG content provides additional prognostic information within both the estrogen receptor (ER) positive patient or estrogen receptor (ER) negative patient groups. There is a correlation between (ER) positive or (ER) negative patients and diploid or aneuploid patients.