Serilmez M., Şen S., Özgür E., Karaman Ş., Gezer U.
Clinical Medicine Insights: Oncology, cilt.20, ss.1-16, 2026 (Hakemli Dergi)
Özet
Background
Non-small cell lung cancer (NSCLC) accounts for the majority of lung cancer cases. Circulating microRNAs (miRNAs) have emerged as promising non-invasive biomarkers for cancer detection. In this exploratory study, we investigated the diagnostic and prognostic value of four circulating miRNAs (miR-107, miR-199-3p, miR-485-5p, and miR-574-5p) in NSCLC, three of which have not previously been examined in this context.
Methods
Serum samples from 58 NSCLC patients and 20 healthy controls were analyzed. Relative expression levels of the target miRNAs were quantified by quantitative RT-PCR. Differences between groups were evaluated with the Mann–Whitney U test, and the diagnostic performance of each miRNA was assessed by receiver operating characteristic (ROC) curve analysis. Univariate and multivariate regression analyses were performed to identify factors associated with survival.
Results
Three of the four miRNAs showed significantly different expression between NSCLC patients and healthy controls: miR-107, miR-199, and miR-485 were all expressed at significantly lower levels in patients (p < 0.001, p < 0.001, and p = 0.002, respectively), whereas miR-574 levels did not differ between groups (p = 0.370). ROC analysis confirmed significant discriminatory power for all four miRNAs (p < 0.001), with miR-107 yielding the highest area under the curve (AUC = 0.952). In multivariate analysis, ECOG performance status and miR-199 emerged as covariates significantly associated with survival.
Conclusion
These exploratory findings indicate that circulating miR-107, miR-199, and miR-485 have strong discriminative potential in NSCLC. Larger validation studies are needed to confirm their utility as non-invasive biomarkers.