Transgenic Investigation of Canola (Brassica napus L.)

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Fidan S., Arıcan E.

Annual Research and Review in Biology, cilt.4, sa.24, ss.4293-4304, 2014 (Scopus)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 4 Sayı: 24
• Basım Tarihi: 2014
• Doi Numarası: 10.9734/arrb/2014/11684
• Dergi Adı: Annual Research and Review in Biology
• Derginin Tarandığı İndeksler: Scopus
• Sayfa Sayıları: ss.4293-4304
• İstanbul Üniversitesi Adresli: Evet

Özet

Investigating transgenic characteristics of Californium, Jura, Elvis and Orkan varieties of canola has been carried out in this study. These plants are widely cultivated in Turkey. The Canola varieties were grown in an established tissue culture and the total DNA isolated. The 35S and pNOS promoter region of the transgene were scanned using classic and multiplex PCR techniques. The transfer of transgenic characteristic to the NAD gene region confirms the accuracy of the PCR technique. This study, has determined that Californium, Jura, Elvis and Orkan varieties of canola consist of transgenic characteristics and all the four varieties have multiple insertion region in terms of CaMV 35S and Pnos promoters.