Evaluation of the performance of rapid antibiotic susceptibility test results using the disk diffusion directly from the positive blood culture bottles

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Kansak N., Adaleti R., Nakipoglu Y., Aksaray S.

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, cilt.39, sa.4, ss.484-488, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.ijmmb.2021.06.008
  • Dergi Adı: INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, CINAHL, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.484-488
  • Anahtar Kelimeler: Blood culture, Rapid antibiotic susceptibility test, EUCAST, Gram negative bacilli, Gram positive cocci, ANTIMICROBIAL SUSCEPTIBILITY, RAST
  • İstanbul Üniversitesi Adresli: Evet

Özet

Purpose: In this study, we aimed to evaluate the compliance of rapid antibiotic susceptibility test (RAST) and
conventional laboratory procedures.
Methods: The RAST was performed directly from the blood cultures of 71 Gram negative bacilli (GNB) and 38
Gram positive cocci (GPC) isolates. The results were evaluated at fourth, sixth and eighth hour. Categorical
agreement (CA), very major error (VME), major error (ME) and minor error (mE) were calculated and compared
with the results of conventional Vitek-2 system.
Results: Categorical agreement was detected 90 in cefotaxime and meropenem at fourth hour in Escherichia coli
isolates. An encourage positive CA results were obtained from meropenem, ceftazidime and ciprofloxacin at the
fourth hour in Klebsiella pneumoniae isolates. CA was compatible in imipenem, ciprofloxacin, gentamicin, and
tobramycin for Pseudomonas aeruginosa at sixth hour. CA was low (<90%) in piperacillin-tazobactam for E. coli
and K. pneumoniae, and meropenem in P. aeruginosa isolates. A good CA (90) with all tested antibiotics were
found at all hours for Acinetobacter baumannii and also very high CA (100%) was detected at sixth and eighth hour
in Staphylococcus aureus isolates. CA remained below the standard criteria at fourth hour in vancomycin and high
level gentamicin, in addition to imipenem at sixth hours in enterococci isolates. VME and ME were not detected
and mE was 12.7% in GNB and 50% in GPC at eighth hour.
Conclusions: EUCAST RAST at eighth hour will be beneficial in urgent patients due to their high CA rate, easy
preparation, inexpensive, and could be performed with the available equipment and personnel.