Spectrofluorimetric determination of nateglinide in pure and pharmaceutical preparations through derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole


Karasakal A., Ulu S.

OPTICS AND SPECTROSCOPY, cilt.116, sa.1, ss.52-55, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 116 Sayı: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1134/s0030400x14010020
  • Dergi Adı: OPTICS AND SPECTROSCOPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.52-55
  • İstanbul Üniversitesi Adresli: Evet

Özet

A sensitive method was developed for the determination of nateglinide in pure and pharmaceutical preparations. The proposed method is based on the derivatization reaction between nateglinide and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole in borate buffer of pH 10. Fluorescent intensity of derivative was measured at 537 nm using an excitation wavelength of 464 nm. The described method was validated and the analytical parameters of linearity, limit of detection, limit of quantification, accuracy, precision (intra- and inter-day) and recovery were evaluated. The assay was linear over the concentration range of 50-500 ng/mL. The proposed method was applied to study of nateglinide in pure and pharmaceutical preparations.