The karyotypes of two Aethionema speciosum subspecies, A. speciosum subsp. speciosum and A. speciosum subsp. compactum, from various phytogeographical areas in Turkey were analyzed. The chromosome counts of the taxa were determined as 2n = 2x = 24 and 2n = 2x = 36, respectively. Karyotypes have mostly median and submedian centromeres. Chromosomes of the examined taxa were analyzed in accordance with some previous cytogenetic reports from a systematic perspective.