Experimental trigeminal glycerol injection in dogs: Histopathological evaluation by light and electron microscopy


Isik N., Pamir M., Benli K., Erbengi A., Erbengi T., Ruacan S.

STEREOTACTIC AND FUNCTIONAL NEUROSURGERY, cilt.79, ss.94-106, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 79 Konu: 2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1159/000070105
  • Dergi Adı: STEREOTACTIC AND FUNCTIONAL NEUROSURGERY
  • Sayfa Sayıları: ss.94-106

Özet

We investigated the effects of percutaneous gasserian glycerol injection in dogs and reviewed the histopathological changes. Experiments were performed in 16 adult healthy mongrel dogs. In group 1 (8 dogs) normal saline and in group 2 (8 dogs) pure glycerol was injected in the right trigeminal ganglion. After these procedures, dogs in each group were sacrificed after 24 h (3 dogs), 7 days (3 dogs), 21 days (2 dogs). The trigeminal ganglion and nerve of both sides were removed by using microsurgical techniques and examined by light and electron microscopy. Group 1: in all sections, nerve cells, myelinated and nonmyelinated fibers revealed normal patterns with slight fibrosis. Group 2: in all sections, myelinated fibers showed disintegration and swelling of the myelin sheath, rupture of axon continuity, destruction of basal lamina, deformation of the myelin-axon relationship by both light microscopy and electron microscopy. The sections examined by electron microscopy also showed axonolysis in nonmyelinated fibers. The changes after 7 and 21 days were less prominent than after 24 h. In the left sides, there are no pathological changes. Glycerol has a neurolytic effect on the dog's trigeminal ganglion. These effects were not specific and selective for myelinated and nonmyelinated nerve fibers. Copyright (C) 2002 S. Karger AG, Basel.