Photochemically prepared monodisperse gold nanoparticles as doxorubicin carrier and its cytotoxicity on leukemia cancer cells


Dizman H. M. , Eroglu G. O. , Kuruca S. E. , ARSU N.

APPLIED NANOSCIENCE, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume:
  • Publication Date: 2020
  • Doi Number: 10.1007/s13204-020-01589-3
  • Title of Journal : APPLIED NANOSCIENCE
  • Keywords: Photochemistry, Gold nanoparticles, Thioxanthone-disulfide, Drug delivery, Anticancer drug, FREE-RADICAL POLYMERIZATION, PHOTOINITIATOR, CHEMOTHERAPY, PARTICLES, MATRIX, TUMOR

Abstract

In this study, a new thioxanthone derivative called 3,3 '-disulfanediylbis(1-hydroxy-9H-thioxanthen-9-one) was utilized in situ for the photochemical synthesis of monodisperse gold nanoparticles, and simple characterization was achieved by formation of a typical surface plasmon resonance band of gold nanoparticles at 535 nm accompanied by a nice pink color. Dynamic light scattering method was employed for determination of the size of the in situ-prepared gold nanoparticles and, with good polydispersity value at around 30 nm, it was in good agreement with the surface plasmon resonance band. After successful preparation of gold nanoparticles, a well-known cancer drug, doxorubicin, at different amounts was loaded onto prepared gold nanoparticles. The adsorbed and released doxorubicin amount onto the gold nanoparticles was calculated by a fluorescence spectrophotometer using a calibration curve. Doxorubicin-loaded gold nanoparticles were examined using a MTT assay for both HL-60 and K562 leukemia cancer cells and HUVEC cells, and 5 mu L doxorubicin-loaded gold nanoparticles significantly reduced cell viability. In normal HUVEC cells, 5 mu L doxorubicin-loaded gold nanoparticles were found less cytotoxic compared to doxorubicin alone. The data obtained in this study showed that gold nanoparticles loaded with 5 mu L doxorubicin are much more effective than doxorubicin used alone in cancer treatment.