Cobalamin Scarcity Modifies Carbon Allocation and Impairs DMSP Production Through Methionine Metabolism in the Haptophyte Microalgae Tisochrysis lutea

ERŞEN A., Henry C., Nicolau E., Berard J., Herve F., Caruana A. M. N., ...Daha Fazla

FRONTIERS IN MARINE SCIENCE, cilt.7, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7
  • Basım Tarihi: 2020
  • Doi Numarası: 10.3389/fmars.2020.569560
  • Dergi Adı: FRONTIERS IN MARINE SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Pollution Abstracts, Directory of Open Access Journals
  • Anahtar Kelimeler: cobalamin, vitamin B-12, haptophyte microalgae, Tisochrysis lutea, DMSP, chemostat, comparative proteomics, metabolism, DIATOM PHAEODACTYLUM-TRICORNUTUM, DIMETHYL SULFIDE, MARINE-BACTERIA, B-VITAMINS, DIMETHYLSULPHONIOPROPIONATE DMSP, DIMETHYLSULFONIOPROPIONATE DMSP, THALASSIOSIRA-PSEUDONANA, PHYTOPLANKTON GROWTH, S-ADENOSYLMETHIONINE, NITROGEN DEPRIVATION
  • İstanbul Üniversitesi Adresli: Evet

Özet

Cobalamin (vitamin B-12) is a cobalt-containing enzymatic cofactor involved in methionine synthesis. Provided only by select bacteria and archaea in marine systems, this vitamin is known to limit primary production in different oceanic areas. Understanding the consequences of cobalamin limitation on phytoplankton physiology is of great interest, notably for cobalamin-dependent haptophytes that significantly contribute to oceanic carbon fixation and sulfur cycle through dimethyl sulfonio propionate (DMSP) synthesis. Here, the effect of cobalamin limitation was compared to nitrogen limitation on the model haptophyte Tisochrysis lutea grown in chemostats, combining comparative proteomics with the analysis of major macromolecules and specific osmolytes. Our results highlight the interconnection of carbon and DMSP metabolisms through the cobalamin-dependent methionine synthesis by showing that cobalamin scarcity impacts the mechanisms of carbon allocation and reduces DMSP quota. Conversely, proline production seemed to anticorrelate with cobalamin availability. In a boarder context, analysis of transcriptomes or genomes of main DMSP producers from different phytoplankton lineages suggests that most of them are cobalamin-dependent, which means that prokaryotic cobalamin synthesis exerts an important control on phytoplankton DMSP production in some regions of the world ocean.