Assessing of antibiotic resistance in the phage-plasmids of Klebsiella pneumoniae and its inhibitory compound
GACETA MEDICA DE MEXICO, cilt.161, ss.346-357, 2025 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 161
- Basım Tarihi: 2025
- Doi Numarası: 10.24875/gmm.25000061
- Dergi Adı: GACETA MEDICA DE MEXICO
- Derginin Tarandığı İndeksler: Scopus, Fuente Academica Plus, EMBASE, MEDLINE, Directory of Open Access Journals, DIALNET
- Sayfa Sayıları: ss.346-357
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- İstanbul Üniversitesi Adresli: Evet
Özet
Background: Antibiotic resistance in Klebsiella pneumoniae poses an increasing risk to human health worldwide.
Objectives: Whole-genome sequence data were used to characterize K. pneumoniae phage-plasmids isolated in different countries.
Materials and methods: Sequences of 15 genetically similar phage-plasmids were selected based on BLASTN analysis. Antibiotic resistance genes were screened using ResFinder, PhageAI, PlasmidFinder, PHASTEST, and PhageGE tools. Phage-plasmid sequences were then analyzed for a stable region in the genome using MAUVE analysis, and the protein of this stable region and its candidate inhibitor molecule were analyzed through molecular docking studies.
Results: Several resistance genes were detected in 7 of the 15 phage-plasmids screened. All plasmids had the Salmonella SSU5 prophage. MAUVE analysis indicated a common stable region encoding replication protein A. Molecular docking studies demonstrated that an interaction occurs between replication protein A and a fabimycin derivative.
Conclusion: Fabimycin could be used to control both phage plasmids and their host, which could affect the severity and incidence of K. pneumoniae infections.