Determination of cyclonite (RDX) in human plasma by high-performance liquid chromatography
Farmaco, cilt.58, sa.6, ss.445-448, 2003 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 58 Sayı: 6
- Basım Tarihi: 2003
- Doi Numarası: 10.1016/s0014-827x(03)00069-7
- Dergi Adı: Farmaco
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, International Pharmaceutical Abstracts
- Sayfa Sayıları: ss.445-448
- İstanbul Üniversitesi Adresli: Evet
Özet
A simple and sensitive HPLC method has been developed for the assay of cyclonite (RDX) in human plasma. The assay involves solid-phase extraction on Tox-clean RC SPE cartridges and isocratic reversed-phase chromatography with diode-array detection. The assay was linear over the concentration range of 0.01-2.0 μg/ml for plasma with a lower limit of detection of 0.005 μg/ml. Both the within-day and day-to-day reproducibilities and accuracies were less than 10.15 and 1.9%, respectively. The method was applied to evaluate RDX concentration in plasma samples obtained from soldiers exposed RDX. © 2003 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.