Akademik Veri Yönetim Sistemi
FRESENIUS ENVIRONMENTAL BULLETIN, cilt.31, sa.9, ss.9867-9877, 2022 (SCI-Expanded)
The present study; seven rice genotypes (Osmancdc-97, Edirne, TG1, TG2, Sur C1, Luna C1 and Reiziq) and positive check Taipei 309 cultivars were used to investigate anther culture efficiency under different in vitro culture environments. For optimization step of calli induction from rice anthers, the spikes were exposed to 10 degrees C cold at 8 days. Afterwards, rice anthers were inoculated on 20 different media compositions. Three Turkish genotypes (Osmancik-97, TG1 and TG2) and Taipei 309 gave positive results in four of twenty culture compositions. The effect of putrescine, gum Arabic and CuSO4 additives and carbon sources, sucrose and maltose, in the calli induction culture media were observed as a genotyped-dependent. N-6 media consist of maltose, 2,4-dichlorophenoxyacetic acid (2,4-D) and gum Arabic showed maximum calli induction rate. Obtained-calli from these four cultivars were put on 10 different solidify Murashige and Skoog (MS) media compositions to obtain plantlets. Two Turkish genotypes (Osmancik-97 and TG1) and Taipei 309 gave positive results in one of ten culture compositions. MS basal salt mixture with 1-naphthaleneacetic acid (NAA), Kinetin, sucrose, myo-inositol and agar gave better results than others. During the optimization phase, irradiation of the rice spikes with low-dose gamma radiation did not give the expected result. In the study to determine the culture ability after optimization, Taipei 309 was more efficient than Osmancik-97 and TG1. It was determined that 23% of the obtained plantlets after flow cytometer analysis were haploid. About 82% of the plantlets transferred to the soil were set to seed. Additionally, gum-Arabic increased anther culture efficiency in rice.