The antimicrobial activity screening of three Verbascum species in Marmara region
PLANTA MEDICA, cilt.78, sa.11, ss.1310, 2012 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 78 Sayı: 11
- Basım Tarihi: 2012
- Doi Numarası: 10.1055/s-0032-1320997
- Dergi Adı: PLANTA MEDICA
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.1310
- İstanbul Üniversitesi Adresli: Evet
Özet
Three Verbascum species in Marmara Region, V. lagurus, V. gnaphalodes and V. xanthophoeniceum,
are collected. The aerial parts were extracted with methanol, chloroform, ethyl acetate and water.
These extracts were compared chromatographically and for their antimicrobial activity with V.
phlomoides and V. densiflorum, which are preferred to be used in phytotherapy.
Two fenolic acids (chlorogenic acid and caffeic acid), three flavonoids (luteolin, luteolin-7-glucoside and
diosmetin-7-glucoside) and three phenylethanoid glycosides (verbascoside, angoroside A and
forsitoside B) were isolated from ethyl acetate extract of V. lagurus, which was shown higher
antimicrobial activity among the other V. lagurus extracts. V. lagurus seems to be similar to medicinal
species. All of the extracts of V. lagurus, showed activity against S. aureus with MIC values of between
156–625mg/L. Ethyl acetate extract, expressed also higher antibacterial activity
against Staphylococcus epidermidis, while only the methanol extract was exhibited antibacterial
activity against Pseudomonas aeruginosa. Antifungal screening result has indicated that methanol,
ethyl acetate and aqueous extracts possess significant antifungal activity againstCandida albicans.
According to these results, the ethyl acetate extract displayed good antimicrobial activity against S.
aureus and C. albicans. This is the first investigation on V. lagurus and V. gnaphalodes, and first
isolation of diosmetin-7- glucoside from Verbascum species.Three Verbascum species in Marmara Region, V. lagurus, V. gnaphalodes and V. xanthophoeniceum,
are collected. The aerial parts were extracted with methanol, chloroform, ethyl acetate and water.
These extracts were compared chromatographically and for their antimicrobial activity with V.
phlomoides and V. densiflorum, which are preferred to be used in phytotherapy.
Two fenolic acids (chlorogenic acid and caffeic acid), three flavonoids (luteolin, luteolin-7-glucoside and
diosmetin-7-glucoside) and three phenylethanoid glycosides (verbascoside, angoroside A and
forsitoside B) were isolated from ethyl acetate extract of V. lagurus, which was shown higher
antimicrobial activity among the other V. lagurus extracts. V. lagurus seems to be similar to medicinal
species. All of the extracts of V. lagurus, showed activity against S. aureus with MIC values of between
156–625mg/L. Ethyl acetate extract, expressed also higher antibacterial activity
against Staphylococcus epidermidis, while only the methanol extract was exhibited antibacterial
activity against Pseudomonas aeruginosa. Antifungal screening result has indicated that methanol,
ethyl acetate and aqueous extracts possess significant antifungal activity againstCandida albicans.
According to these results, the ethyl acetate extract displayed good antimicrobial activity against S.
aureus and C. albicans. This is the first investigation on V. lagurus and V. gnaphalodes, and first
isolation of diosmetin-7- glucoside from Verbascum species.