Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation

Karakaş Ö., UÇARLI C., Gözükirmizi N., Gürel F.

Journal of Applied Biological Sciences, vol.5, no.1, pp.55-58, 2011 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 5 Issue: 1
  • Publication Date: 2011
  • Journal Name: Journal of Applied Biological Sciences
  • Journal Indexes: Scopus
  • Page Numbers: pp.55-58
  • Istanbul University Affiliated: Yes

Abstract

This study describes a protocol for Agrobacterium-mediated transformation of barley mature embryos. Cultured embryos were co-cultivated
by EHA 105 strain carrying pCambia2301 binary vector and assayed for transient GUS expression. After 3 days of transformation, GUS expressing
spots have been observed over 20.3% of total embryos. Among the tested several pre-treatments, infiltration in a vacuum chamber (400 mm Hg for
20 min) and infection by AGL-1 strain instead of EHA 105 increased the numbers of embryos with GUS staining significantly (p<0.05). PCR and
Southern analyses showed that transformation occured in some of the T0 plants which were also tolerant to selective conditions.