Apoptosis-inducing Effect of a Palladium(II) Complex-[PdCl(terpy)](sac).2H2O] on Ehrlich Ascites Carcinoma (EAC) in Mice

Ikitimur-Armutak E., Ulukaya E., Gurel-Gurevin E., Yaylim İ., Isbilen-Basok B., Sennazli G., ...Daha Fazla

In vivo (Athens, Greece), cilt.30, sa.4, ss.457-464, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 4
  • Basım Tarihi: 2016
  • Dergi Adı: In vivo (Athens, Greece)
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.457-464
  • Anahtar Kelimeler: Pd(II) complex, EAC, breast cancer, apoptosis, in vivo, BREAST-CANCER CELLS, NUCLEAR ANTIGEN IMMUNOHISTOCHEMISTRY, INSULIN-RECEPTOR SUBSTRATE-1, GENE BAX-ALPHA, SACCHARINATE COMPLEX, IN-VITRO, TUMOR-GROWTH, DEATH, PHOSPHORYLATION, TERPYRIDINE
  • İstanbul Üniversitesi Adresli: Evet

Özet

Copyright © 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.BACKGROUND/AIM: New compounds for cancer treatment are needed due to persistenly unsatisfactory management of cancer. [PdCl(terpy)](sac)·2H2O] (sac=saccharinate, and terpy=2,2':6',2"-terpyridine) is a compound synthesized for this purpose. We investigated its anti-proliferative and pro-apoptotic effects on Ehrlich Ascites Carcinoma (EAC) in vivo.MATERIALS AND METHODS: 42 Balb-c female mice were subcutaneously (s.c.) injected with EAC cells (1st day) and then randomly divided into 5 groups: control (0.9% NaCl), complex (2 mg/kg), complex (3 mg/kg) cisplatin (4 mg/kg) and paclitaxel (12.5 mg/kg). On the 5th and 12th day animals were drug administrated. At 14th day, animals were sacrificed. Expression of cell death and/or cell cycle-related markers (Bcl-2, Bax, active caspase-3, p53, PCNA) and apoptosis were investigated immunohisto-chemically. Survival-related markers (Akt, GSK-3β, IGF-1R, IR, IRS-1, p70S6K, PRAS40) were evaluated by luminex analysis.RESULTS: Expression of p53, PCNA, Bcl-2 was found decreased (p<0.001) and that of active caspase-3, Bax, and apoptotic cells was found increased (p<0.001) in all groups. The survival-related markers did not show any statistical difference in complex groups.CONCLUSION: The Pd(II)-complex seems to have a strong anticancer activity on EAC by inducing apoptosis via both suppression of proliferation and activation of apoptosis in vivo, similar to the effects of cisplatin and paclitaxel.