Kuruca D. S., Akgün Dar K., Kapucu A., Özerkan D.

Journal of Istanbul Faculty of Medicine, vol.83, no.3, pp.234-241, 2019 (Peer-Reviewed Journal)


Abstract :

Objective: Environmental pollution and nutrition with plants and animals exposed to chemical substances such as 1,2-Dimethylhydrazine (DMH) causes many diseases. Colon cancer is one of them. DMH has been found to accumulate in the gastrointestinal tract, impairing the functions of its organs and causing cancer. Zeolite is a volcanic mineral which contains aluminum silicate, which is used in many areas due to its absorption and ion exchange capability, and which has had widespread medical use in recent years. Clinoptilolite, the most well-known species of zeolite, proved to be harmless, did not enter the blood when administered orally to humans and was excreted as a faeces from the gastrointestinal tract. In addition, clinoptilolite administered orally to humans passes through the gastrointestinal tract without dissolving in the blood. Material and Method: The aim of this study was to investigate the effects of clinoptilolite nutrition on colon cancer induced by DMH in rats. The rats were divided into four experimental groups which were control, clinoptilolite, DMH, and clinoptilolite + DMH using a total of 46 Wistar albino rats. Rats of DMH and clinoptilolite + DMH groups were injected withDMH subcutaneously once a week for 16 weeks at a dose of 20 mg/kg and also clinoptilolite and clinoptilolite+DMH groups were fed a diet with 50% clinoptilolite for eight months. Results: Molecular changes were examined by immunohistochemical staining in colon tissue samples with B-catenin antibody specific for Wnt-Bcatenin signaling pathway, which is common in colon cancer. The reaction intensity of B-catenin was determined as DMH clinoptilolite control DMH + clinoptilolite, respectively. Conclusion: We may think that clinoptilolite reduces the poten tial toxic effects of DMH by decreasing cell proliferation.